Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH0-87 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 44 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human ERCC8 aa 1-396 / 396. |
Positive control: | 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate. |
Subcellular location: | Nucleus, Nucleus matrix. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q13216 Human |
Alternative names: | CKN1 Cockayne syndrome type A Cockayne syndrome WD repeat protein CSA CSA DNA excision repair protein ERCC-8 DNA excision repair protein ERCC8 ERCC 8 ERCC8 ERCC8_HUMAN excision repair cross-complementing rodent repair deficiency, complementation group 8 |
Fig1:
Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (HA721522) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 55 kDa Exposure time: 1 minute 32 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721522) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |