BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-88]
cat.: HA721523
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH0-88
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 15 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BLBP aa 1-132 / 132.
Positive control: Human brain tissue lysate, human cerebellum tissue lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O15540 Human
Alternative names: B FABP B-FABP BFABP BLBP Brain lipid binding protein Brain lipid-binding protein Brain-type fatty acid-binding protein DKFZp547J2313 FABP 7 FABP7 FABP7_HUMAN FABPB Fatty Acid Binding Protein 7 Fatty acid binding protein 7 brain Fatty acid binding protein brain Fatty acid-binding protein 7 Fatty acid-binding protein, brain Mammary derived growth inhibitor related Mammary-derived growth inhibitor related MRG OTTHUMP00000017119
Images
HA721523_1.jpg Fig1: Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA721523) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Human cerebellum tissue lysate
Lane 3: Mouse lung tissue lysate (negative)
Lane 4: MCF7 cell lysate (negative)

Lysates/proteins at 30 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721523) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.