BLBP Recombinant Rabbit Monoclonal Antibody [PSH0-89]
cat.: HA721524
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BLBP aa 1-132 / 132. |
| Positive control: | Human brain tissue lysate, human cerebellum tissue lysate, human brain tissue, human brain glioma tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:3,000-1:5,000 |
| Uniprot #: | SwissProt: O15540 Human |
| Alternative names: | B FABP B-FABP BFABP BLBP Brain lipid binding protein Brain lipid-binding protein Brain-type fatty acid-binding protein DKFZp547J2313 FABP 7 FABP7 FABP7_HUMAN FABPB Fatty Acid Binding Protein 7 Fatty acid binding protein 7 brain Fatty acid binding protein brain Fatty acid-binding protein 7 Fatty acid-binding protein, brain Mammary derived growth inhibitor related Mammary-derived growth inhibitor related MRG OTTHUMP00000017119 |
Images
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Fig1:
Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA721524) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: MCF7 cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721524) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-BLBP antibody (HA721524) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain glioma tissue with Rabbit anti-BLBP antibody (HA721524) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-BLBP antibody (HA721524) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721524) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.