HIV1 p24 Recombinant Rabbit Monoclonal Antibody [PS01-35]
cat.: HA721527
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | HIV1 |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PS01-35 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within HIV1 p24 aa 133-363 (HA210738). |
| Positive control: | Recombanint HIV1 p24 protein (HA210738). |
| Subcellular location: | Virion. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-31] to HIV1 p24 (Detecor) (HA721528) and Recombinant HIV1 p24 protein (HA210738) as the standard. The reference range value is 39.06-5,000 pg/mL. |
| Uniprot #: | SwissProt: P12497 HIV1 |
| Alternative names: | CA Capsid protein p24 HIV-1 Gag p24 HIV1gp1 Human immunodeficiency virus 1 Human immunodeficiency virus type 1 p24 |
Images
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Fig1:
Sandwich ELISA analysis of HIV1 p24 protein matched pair antibodies Capture: HA721527, HIV1 p24 Rabbit mAb [PS01-35] Detector: HA721528, HIV1 p24 Rabbit mAb [PS01-31] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721527) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant HIV1 p24 protein (HA210738) starting from 5,000 pg/mL to 0 pg/mL and detect antibody (HA721528, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.