FUNDC1 Recombinant Rabbit Monoclonal Antibody [PSH0-92]
cat.: HA721530
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N terminal human FUNDC1. |
| Positive control: | 293T over-expressed FUNDC1 cell lysate, HepG2 cell lysates, rat brain tissue. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50 |
| Uniprot #: | SwissProt: Q8IVP5 Human | Q5BJS4 Rat |
| Alternative names: | FUN14 domain containing 1 FUN14 domain containing protein 1 FUN14 domain-containing protein 1 FUND1_HUMAN fundc1 |
Images
|
Fig1:
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution. Lane 1: 293T over-expressed FUNDC1 cell lysate Lane 2: 293T control cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of FUNDC1 on HepG2 cell lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FUNDC1 antibody (HA721530) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721530) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.