Laminin 2 alpha Recombinant Rabbit Monoclonal Antibody [PSH0-94]
cat.: HA721532
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH0-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 344 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Laminin 2 alpha 1,151-1,400 / 3,122. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human heart tissue, mouse heart tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P24043 Human | Q60675 Mouse Entrez Gene: 309368 Rat |
| Alternative names: | LAMA 2 LAMA2 LAMA2_HUMAN Laminin alpha 2 (merosin congenital muscular dystrophy) Laminin alpha 2 Laminin alpha 2 chain Laminin alpha 2 subunit Laminin M Laminin M chain Laminin subunit alpha-2 Laminin-12 subunit alpha Laminin-2 subunit alpha Laminin-4 subunit alpha LAMM Merosin heavy chain |
Images
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Fig1:
Western blot analysis of Laminin 2 alpha on different lysates with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 344 kDa Observed band size: 344 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721532) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Laminin 2 alpha with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721532, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.