CD9 Recombinant Rabbit Monoclonal Antibody [PSH0-95]
cat.: HA721533
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD9 aa 196-228 / 228. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, SW480 cell lysate, BT-20 cell lysate, human colon carcinoma tissue, human lung carcinoma tissue. |
| Subcellular location: | Cell membrane, Membrane, Secreted, extracellular exosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P21926 Human |
| Alternative names: | Tetraspanin 29 5H9 5H9 antigen Antigen defined by monoclonal 602 29 Antigen defined by monoclonal 60229 BA-2/p24 antigen BA2 BTCC 1 BTCC1 CD9 CD9 antigen CD9 antigen p24 CD9 molecule CD9_HUMAN Cell growth inhibiting gene 2 protein Cell growth-inhibiting gene 2 protein DRAP 27 DRAP27 GIG2 Growth inhibiting gene 2 protein Leukocyte antigen MIC3 MIC3 Motility related protein Motility-related protein MRP 1 MRP-1 MRP1 p24 p24 antigen Tetraspanin-29 Tspan 29 Tspan-29 TSPAN29 |
Images
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Fig1:
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: MCF7 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: Lane 1-4 (left): 53 seconds; Lane 1-4 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: SW480 cell lysate (10 µg/Lane) Lane 3: BT-20 cell lysate (10 µg/Lane) Lane 4: MCF7 cell lysate (10 µg/Lane) Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/2,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si CD9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD9 antibody (HA721533) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721533) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-CD9 antibody (HA721533) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721533) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.