SEC62 Recombinant Rabbit Monoclonal Antibody [JE62-80]
cat.: HA721534
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, FC |
| Clonality: | Monoclonal |
| Clone number: | JE62-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SEC62 aa256-350. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, K-562 cell lysate, A431 cell lysate, HEK-293 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, HeLa. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC FC |
1:1,000 1:1,000-1:3,000 1:1,000 |
| Uniprot #: | SwissProt: Q99442 Human | Q8BU14 Mouse Entrez Gene: 294912 Rat |
| Alternative names: | Dtrp1 FLJ32803 hTP-1 HTP1 Membrane protein SEC62, S.cerevisiae, homolog of OTTHUMP00000213390 sec62 SEC62 homolog (S. cerevisiae) SEC62_HUMAN TLOC1 TP-1 Translocation protein 1 Translocation protein sec62 |
Images
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Fig1:
Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721534) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: HEK-293 cell lysate Lane 7: PANC-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721534) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of HeLa cells labeling SEC62. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721534, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.