Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | PS01-06 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 22 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within full length protein. |
Positive control: | A431 cell lysate, SiHa cell lysate, A431. |
Subcellular location: | Cytoplasm, Secreted. |
Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
Uniprot #: | SwissProt: Q14116 Human |
Alternative names: | Iboctadekin IFN gamma inducing factor IFN-gamma-inducing factor IGIF IL 1 gamma IL 18 IL 1g IL-1 gamma IL-18 IL1 gamma IL18 IL18 protein IL18_HUMAN IL1F4 IL1g IL1gamma ILIF4 Interferon gamma inducing factor Interferon gamma-inducing factor Interleukin 1 gamma Interleukin 18 (interferon-gamma-inducing factor) Interleukin 18 Interleukin-1 gamma Interleukin-18 Interleukin18 MGC12320 |
Fig1:
Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA721537) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721537) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of A431 cells labeling IL-18. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721537, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |