Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE38-13 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 97 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human TYRO3 aa 841-890 / 890. |
Positive control: | HeLa cell lysate, HCT 116 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q06418 Human | P55146 Rat |
Alternative names: | Brt BYK DTK Etk-2 protein tyrosine kinase 3 Rek RSE SKY Tif tyro3 TYRO3 protein tyrosine kinase TYRO3_HUMAN Tyrosine-protein kinase byk Tyrosine-protein kinase DTK Tyrosine-protein kinase receptor TYRO3 Tyrosine-protein kinase RSE Tyrosine-protein kinase SKY Tyrosine-protein kinase TIF |
Fig1:
Western blot analysis of TYRO3 on different lysates with Rabbit anti-TYRO3 antibody (HA721539) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Human brain tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721539) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |