Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JE32-01 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 8 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Trefoil Factor 3 aa 31-80 / 80. |
Positive control: | Human small intestine tissue lysates, human small intestine tissue, HT-29. |
Subcellular location: | Secreted > extracellular space > extracellular matrix > apical lamina. |
Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:500-1:1,000 |
Uniprot #: | SwissProt: Q07654 Human |
Alternative names: | hITF hP1.B Intestinal trefoil factor ITF mITF OTTMUSP00000021729 P1B Polypeptide P1.B TFF3 TFF3_HUMAN TFI TREFOIL Trefoil factor (intestinal) Trefoil factor 3 |
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Fig1:
Western blot analysis of Trefoil Factor 3 on human small intestine tissue lysates with Rabbit anti-Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 8 kDa Observed band size: 11 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721541) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HT-29 cells labeling Trefoil Factor 3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721541, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |