| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE66-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CXCR6 aa 1-100. |
| Positive control: | JAR cell lysate, SW480 cell lysate, human placenta tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O00574 Human |
| Alternative names: | bonzo C X C chemokine receptor type 6 C-X-C chemokine receptor type 6 CD 186 CD186 CD186 antigen CDw186 Chemokine (C X C motif) receptor 6 Chemokine C X C motif receptor 6 Chemokine CXC motif receptor 6 Chemokine receptor 6 CXC chemokine receptor type 6 CXC R6 CXC-R6 CXCR 6 CXCR-6 CXCR6 CXCR6_HUMAN G protein coupled receptor G protein coupled receptor bonzo G protein coupled receptor STRL 33 G protein coupled receptor STRL33 G protein coupled receptor TYMSTR G-protein coupled receptor bonzo G-protein coupled receptor STRL33 STRL 33 STRL33 TYMSTR |
|
Fig1:
Western blot analysis of CXCR6 on different lysates with Rabbit anti-CXCR6 antibody (HA721542) at 1/1,000 dilution. Lane 1: JAR cell lysate (10 µg/Lane) Lane 2: SW480 cell lysate (20 µg/Lane) Lane 3: Human placenta tissue lysate (30 µg/Lane) Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721542) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |