Serotonin transporter Recombinant Rabbit Monoclonal Antibody [JE34-53]
cat.: HA721544
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE34-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Serotonin transporter aa 38-87 / 630. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse lung tissue lysate, HepG2. |
| Subcellular location: | Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P31645 Human | Q60857 Mouse | P31652 Rat |
| Alternative names: | 5 HTT 5 HTTLPR 5 hydroxytryptamine (serotonin) transporter 5 hydroxytryptamine transporter 5HT transporter 5HTT hSERT HTT Na+/Cl- dependent serotonin transporter OCD1 SC6A4_HUMAN Serotonin transporter 1 SERT SERT1 Slc6a4 Sodium dependent serotonin transporter Sodium-dependent serotonin transporter Solute carrier family 6 (neurotransmitter transporter) member 4 solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 Solute carrier family 6 member 4 |
Images
|
Fig1:
Western blot analysis of Serotonin transporter on different lysates with Rabbit anti-Serotonin transporter antibody (HA721544) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 75 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721544) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HepG2 cells labeling Serotonin transporter. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721544, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.