Netrin 1 Recombinant Rabbit Monoclonal Antibody [JE38-87]
cat.: HA721545
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-87 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Netrin 1 aa 505-604 / 604. |
| Positive control: | Human small intestine tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate. |
| Subcellular location: | Secreted > extracellular space > extracellular matrix. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95631 Human | O09118 Mouse | Q924Z9 Rat |
| Alternative names: | epididymis tissue protein Li 131P NET1_HUMAN Netrin 1, mouse, homolog of Netrin 1-like Netrin-1 Netrin1 Ntn 1 NTN 1L NTN1 NTN1L Unc6 |
Images
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Fig1:
Western blot analysis of Netrin 1 on different lysates with Rabbit anti-Netrin 1 antibody (HA721545) at 1/1,000 dilution. Lane 1: Human small intestine tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721545) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.