Loricrin Recombinant Rabbit Monoclonal Antibody [JE40-03]
cat.: HA721547
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: JE40-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Loricrin aa 1-100.
Positive control: Human skin tissue, mouse skin tissue, rat skin tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  IHC-P
  IF-Tissue

1:200-1:1,000
1:200
Uniprot #: SwissProt: P23490 Human | P18165 Mouse
Entrez Gene: 502541 Rat
Alternative names: LOR LOR protein LORI_HUMAN Loricrin LRN MGC111513 OTTHUMP00000015823
Images
HA721547_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Loricrin antibody (HA721547) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721547) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721547_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Loricrin antibody (HA721547) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721547) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721547_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Loricrin antibody (HA721547) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721547) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721547_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Loricrin with Rabbit anti-Loricrin antibody (HA721547) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721547, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.