MAGOH Recombinant Rabbit Monoclonal Antibody [JE33-53]
cat.: HA721548
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE33-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human MAGOH aa 97-146 / 146.
Positive control: HCT 116 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, mouse brain tissue, rat brain tissue.
Subcellular location: Nucleus, Nucleus speckle, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200-1:1,000
Uniprot #: SwissProt: P61326 Human | P61327 Mouse | Q27W02 Rat
Alternative names: Mago nashi homolog proliferation associated (Drosophila) Mago nashi protein homolog magoh MAGOHA MGN_HUMAN Protein mago nashi homolog
Images
HA721548_1.jpg Fig1: Western blot analysis of MAGOH on different lysates with Rabbit anti-MAGOH antibody (HA721548) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721548) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721548_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAGOH antibody (HA721548) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721548_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAGOH antibody (HA721548) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721548) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.