PTBP2 Recombinant Rabbit Monoclonal Antibody [JE66-55]
cat.: HA721549
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE66-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PTBP2 aa 482-531 / 531. |
| Positive control: | Neuro-2a cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human brain tissue, mouse hippocampus tissue, rat brain tissue, mouse testis tissue, HeLa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UKA9 Human | Q91Z31 Mouse | Q66H20 Rat |
| Alternative names: | brPTB FLJ34897 MIBP Neural polypyrimidine tract binding protein Neural polypyrimidine tract-binding protein Neurally enriched homolog of PTB Neurally-enriched homolog of PTB nPTB 5 nPTB 6 nPTB 7 nPTB 8 nPTB nPTB5 nPTB6 nPTB7 nPTB8 Polypyrimidine tract binding protein 2 Polypyrimidine tract-binding protein 2 PTB PTB like PTB like protein PTB-like protein PTBLP PTBP 2 Ptbp2 PTBP2_HUMAN Splicing regulator |
Images
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Fig1:
Western blot analysis of PTBP2 on different lysates with Rabbit anti-PTBP2 antibody (HA721549) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (15 µg/Lane) Lane 2: Mouse testis tissue lysate (30 µg/Lane) Lane 3: Rat testis tissue lysate (30 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721549) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PTBP2 antibody (HA721549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-PTBP2 antibody (HA721549) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721549) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PTBP2 antibody (HA721549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PTBP2 antibody (HA721549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HeLa cells labeling PTBP2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721549, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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