FOLR1 Recombinant Rabbit Monoclonal Antibody [JE31-40]
cat.: HA721551
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE31-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FOLR1 aa 21-70 / 257. |
| Positive control: | HeLa cell lysate, JAR cell lysate, rat testis tissue lysate, A549 cell lysate, SK-OV-3 cell lysate, rat brain tissue lysates. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P15328 Human Entrez Gene: 171049 Rat |
| Alternative names: | adult Adult folate binding protein Adult folate-binding protein FBP Folate Binding Protein Folate Receptor 1 Adult Folate receptor 1 Folate Receptor 1 Precursor Folate receptor adult Folate receptor alpha Folate receptor FOLR FOLR1 FOLR1_HUMAN FR alpha FR-alpha FRalpha KB cells FBP MOV18 Ovarian cancer associated antigen Ovarian tumor associated antigen Ovarian tumor associated antigen MOv18 Ovarian tumor-associated antigen MOv18 |
Images
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Fig1:
Western blot analysis of FOLR1 on different lysates with Rabbit anti-FOLR1 antibody (HA721551) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: JAR cell lysate (20 µg/Lane) Lane 3: Rat testis tissue lysate (40 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: SK-OV-3 cell lysate (20 µg/Lane) Predicted band size: 30 kDa Observed band size: 37~70 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721551) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of FOLR1 on rat brain tissue lysates with Rabbit anti-FOLR1 antibody (HA721551) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721551) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.