Rab11A Recombinant Rabbit Monoclonal Antibody [JE42-00]
cat.: HA721552
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE42-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Rab11A aa 117-216 / 216. |
| Positive control: | HeLa cell lysate, A549 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue lysate, human small intestine tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P62491 Human | P62492 Mouse | P62494 Rat |
| Alternative names: | MGC1490 Rab 11 Rab 11A RAB 11A, member oncogene family Rab-11 RAB11 A RAB11 Rab11a RAB11A member RAS oncogene family Ras related protein Rab 11A Ras related protein Rab11A Ras-related protein Rab-11A RB11A_HUMAN YL 8 YL8 |
Images
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Fig1:
Western blot analysis of Rab11A on different lysates with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: Neuro-2a cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721552) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.