Rab11A Recombinant Rabbit Monoclonal Antibody [JE42-00]
cat.: HA721552
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE42-00
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Rab11A aa 117-216 / 216.
Positive control: HeLa cell lysate, A549 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue lysate, human small intestine tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: P62491 Human | P62492 Mouse | P62494 Rat
Alternative names: MGC1490 Rab 11 Rab 11A RAB 11A, member oncogene family Rab-11 RAB11 A RAB11 Rab11a RAB11A member RAS oncogene family Ras related protein Rab 11A Ras related protein Rab11A Ras-related protein Rab-11A RB11A_HUMAN YL 8 YL8
Images
HA721552_1.jpg Fig1: Western blot analysis of Rab11A on different lysates with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Neuro-2a cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (40 µg/Lane)
Lane 7: Rat testis tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721552_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721552) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.