GFPT1 Recombinant Rabbit Monoclonal Antibody [JE43-68]
cat.: HA721554
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE43-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 79 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GFPT1 aa 600-699.
Positive control: HeLa cell lysate, 293T cell lysate, mouse testis tissue lysate, rat spleen tissue lysate, human colon carcinoma tissue, mouse kidney tissue.
Subcellular location: Cytosol
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200-1:1,000
Uniprot #: SwissProt: Q06210 Human | P47856 Mouse | P82808 Rat
Alternative names: CMS12 CMSTA1 D-fructose-6-phosphate amidotransferase 1 GFA GFAT 1 GFAT GFAT1 GFAT1m GFPT Gfpt1 GFPT1_HUMAN GFPT1L Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 Glutamine--fructose-6-phosphate transaminase 1 Glutamine:fructose 6 phosphate amidotransferase 1 Hexosephosphate aminotransferase 1 MSLG
Images
HA721554_1.jpg Fig1: Western blot analysis of GFPT1 on different lysates with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 79 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721554) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721554_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721554) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721554_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GFPT1 antibody (HA721554) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721554) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721554_4.jpg Fig4: Western blot analysis of GFPT1 on different lysates with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si GFPT1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 79 kDa
Observed band size: 75 kDa

Exposure time: 1 minute 7 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721554) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.