DGAT1 Recombinant Rabbit Monoclonal Antibody [JE34-88]
cat.: HA721555
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DGAT1 aa 1-150 / 488. |
| Positive control: | Human liver tissue lysate, MCF7 cell lysate, human liver tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O75907 Human |
| Alternative names: | ACAT related gene product 1 ACAT-related gene product 1 Acyl coenzyme A:cholesterol acyltransferase related gene 1 Acyl-CoA retinol O-fatty-acyltransferase Acyl-CoA:diacylglycerol acyltransferase ARAT ARGP1 C75990 D15Ertd23e Dgat DGAT1 DGAT1_HUMAN Diacylglycerol O acyltransferase 1 Diacylglycerol O-acyltransferase 1 DIAR7 Diglyceride acyltransferase EC 2.3.1.20 hCG_24006 MGC139064 Retinol O fatty acyltransferase |
Images
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Fig1:
Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 µg/Lane. Predicted band size: 55 kDa Observed band size: 48 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si DGAT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 48 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721555) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.