CD83 Recombinant Rabbit Monoclonal Antibody [JE33-61]
cat.: HA721556
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE33-61
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD83 aa 51-100 (Extracellular).
Positive control: Raji cell lysate, Jurkat cell lysate, U-937 cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: Q01151 Human
Alternative names: B cell activation 45kDa cell surface glycoprotein Ig superfamily B cell activation protein B-cell activation protein BL11 BL11 PEN CD83 CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) CD83 antigen CD83 molecule CD83_HUMAN Cell surface glycoprotein Cell surface protein HB15 HB15 hCD83
Images
HA721556_1.jpg Fig1: Western blot analysis of CD83 on different lysates with Rabbit anti-CD83 antibody (HA721556) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U-937 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 35-45 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.