MCM4 Recombinant Rabbit Monoclonal Antibody [JE38-76]
cat.: HA721557
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE38-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MCM4 aa 800-863. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, HL-60 cell lysate, human tonsil tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P33991 Human |
| Alternative names: | 19G AI325074 AU045576 CDC 21 CDC 54 CDC21 CDC21 homolog CDC21, S. pombe, homolog of CDC54 Cell division cycle 21, S. pombe, homolog of DNA replication licensing factor MCM 4 DNA replication licensing factor MCM4 hCdc 21 hCdc21 Homolog of S. pombe cell devision cycle 21 KIAA4003 mcdc21 MCM 4 MCM 4 minichromosome maintenance deficient 4 mcm4 MCM4 minichromosome maintenance deficient 4 MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) MCM4_HUMAN Mcmd4 MGC33310 Minichromosome maintenance 4 Minichromosome maintenance complex component 4 Minichromosome maintenance deficient (S. cerevisiae) 4 Minichromosome maintenance deficient 4 Minichromosome maintenance deficient 4 homolog (S. cerevisiae) Minichromosome maintenance, S. cerevisiae, homolog of, 4 mKIAA4003 NKCD NKGCD P1 CDC21 P1-CDC21 P1CDC21 |
Images
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Fig1:
Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (HA721557) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721557) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MCM4 antibody (HA721557) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721557) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.