FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52]
cat.: HA721558
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE38-52
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human FOXL2 aa 1-50 / 376.
Positive control: THP-1 cell lysate, K-562 cell lysate, human ovary tissue, mouse ovary tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: P58012 Human | O88470 Mouse
Alternative names: Blepharophimosis Blepharophimosis epicanthus inversus and ptosis 1 Blepharophimosis epicanthus inversus and ptosis BPES 1 BPES BPES1 Epicanthus inversus and ptosis 1 Forkhead box L2 Forkhead box protein L2 Forkhead transcription factor FOXL2 FOX L2 FOXL 2 FOXL2 FOXL2_HUMAN PFRK PINTO PITUITARY FORKHEAD FACTOR POF 3 POF3
Images
HA721558_1.jpg Fig1: Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: K-562 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 45 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721558) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721558_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721558_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.