CRMP2 Recombinant Rabbit Monoclonal Antibody [JE31-53]
cat.: HA721560
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE31-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CRMP2 aa 473-572 / 572. |
| Positive control: | U-87 MG cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y. |
| Subcellular location: | Cytoplasm. Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q16555 Human | O08553 Mouse | P47942 Rat |
| Alternative names: | Collapsin response mediator protein 2 Collapsin response mediator protein Collapsin response mediator protein hCRMP 2 CRAM CRMP 2 CRMP-2 CRMP2 DHPRP 2 DHPRP2 Dihydropyrimidinase 2 Dihydropyrimidinase like 2 Dihydropyrimidinase like 2 long form Dihydropyrimidinase related protein 2 Dihydropyrimidinase-related protein 2 DPYL 2 DPYL2 DPYL2_HUMAN DPYSL 2 Dpysl2 DRP-2 DRP2 Musunc 33 Musunc33 N2A3 TOAD 64 TOAD64 ULIP 2 protein ULIP-2 Ulip2 Unc-33-like phosphoprotein 2 |
Images
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Fig1:
Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (HA721560) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (10 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (10 µg/Lane) Lane 5: Mouse brain tissue lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (10 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721560) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling CRMP2 with Rabbit anti-CRMP2 antibody (HA721560) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRMP2 antibody (HA721560) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling CRMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721560, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.