Phospholipase C beta 3/PLCB3 Recombinant Rabbit Monoclonal Antibody [JE39-14]
cat.: HA721561
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE39-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 139 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Phospholipase C beta 3/PLCB3 aa 1,161-1,210 / 1,234. |
| Positive control: | K-562 cell lysate, MCF7 cell lysate, HeLa cell lysate, HCT 116 cell lysate, SH-SY5Y cell lysate, human small intestine tissue. |
| Subcellular location: | Cytoplasm. Membrane. Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: Q01970 Human |
| Alternative names: | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-3 Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta-3 Phospholipase C beta 3 Phospholipase C beta 3 (phosphatidylinositol specific) Phospholipase C-beta-3 PLC beta 3 PLC-beta-3 Plcb3 PLCB3_HUMAN PLCB3 |
Images
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Fig1:
Western blot analysis of Phospholipase C beta 3/PLCB3 on different lysates with Rabbit anti-Phospholipase C beta 3/PLCB3 antibody (HA721561) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721561) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Phospholipase C beta 3/PLCB3 antibody (HA721561) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721561) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.