GLB1 / Beta-galactosidase Recombinant Rabbit Monoclonal Antibody [JE40-77]
cat.: HA721562
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GLB1 aa 628-677 / 677. |
| Positive control: | SK-Br-3 cell lysate, Saos-2 cell lysate, U-87 MG cell lysate, MCF7 cell lysate, HepG2 cell lysate, K-562 cell lysate, Daudi cell lysate, NIH/3T3 cell lysate, Rat brain tissue lysate, mouse placenta tissue, SK-Br-3. |
| Subcellular location: | Lysosome and Cytoplasm. |
| Recommended Dilutions:
WB FC IHC-P |
1:1,000 1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P16278 Human | P23780 Mouse Entrez Gene: 316033 Rat |
| Alternative names: | Acid beta galactosidase Acid beta-galactosidase Beta galactosidase 1 Beta galactosidase Beta-galactosidase BGAL_HUMAN EBP EBP, included Elastin receptor 1 (67kD) Elastin receptor 1 67kDa Elastin receptor 1 Elastin receptor 1, included Elastin-binding protein, included ELNR1 Galactosidase beta 1 GLB 1 GLB1 Lactase MPS4B S-GAL, included |
Images
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Fig1:
Western blot analysis of GLB1 / Beta-galactosidase on different lysates with Rabbit anti-GLB1 / Beta-galactosidase antibody (HA721562) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Saos-2 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: Daudi cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 76 kDa Observed band size: 76/100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721562) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-GLB1 / Beta-galactosidase antibody (HA721562) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721562) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of SK-Br-3 cells labeling GLB1 / Beta-galactosidase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721562, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.