Phospholipase A2 Recombinant Rabbit Monoclonal Antibody [JE41-02]
cat.: HA721565
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE41-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Phospholipase A2 aa 95-144 / 144. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HepG2 cell lysate, rat liver tissue lysate, rat spleen tissue lysate, human liver tissue lysate, mouse liver tissue lysate, mouse spleen tissue lysate, mouse spleen tissue. |
| Subcellular location: | Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane, Secreted |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P14555 Human | P31482 Mosue | P14423 Rat |
| Alternative names: | EC 3.1.1.4 GIIC sPLA2 Group IIA phospholipase A2 membrane associated Modifier of MIN 1 mouse homolog MOM1 Non-pancreatic secretory phospholipase A2 NPS PLA2 NPS-PLA2 PA2GA_HUMAN Phosphatidylcholine 2-acylhydrolase 2A Phosphatidylcholine 2acylhydrolase Phospholipase A2 Phospholipase A2 group IIA (platelets, synovial fluid) Phospholipase A2 membrane associated Phospholipase A2 polypeptide B Phospholipase A2 synovial PLA2 PLA2B PLA2G2A PLA2L PLA2S PLAS1 RASF A sPLA2 |
Images
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Fig1:
Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (40 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.