Phospholipase A2 Recombinant Rabbit Monoclonal Antibody [JE41-02]
cat.: HA721565
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE41-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Phospholipase A2 aa 95-144 / 144.
Positive control: HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HepG2 cell lysate, rat liver tissue lysate, rat spleen tissue lysate, human liver tissue lysate, mouse liver tissue lysate, mouse spleen tissue lysate, mouse spleen tissue.
Subcellular location: Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane, Secreted
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: P14555 Human | P31482 Mosue | P14423 Rat
Alternative names: EC 3.1.1.4 GIIC sPLA2 Group IIA phospholipase A2 membrane associated Modifier of MIN 1 mouse homolog MOM1 Non-pancreatic secretory phospholipase A2 NPS PLA2 NPS-PLA2 PA2GA_HUMAN Phosphatidylcholine 2-acylhydrolase 2A Phosphatidylcholine 2acylhydrolase Phospholipase A2 Phospholipase A2 group IIA (platelets, synovial fluid) Phospholipase A2 membrane associated Phospholipase A2 polypeptide B Phospholipase A2 synovial PLA2 PLA2B PLA2G2A PLA2L PLA2S PLAS1 RASF A sPLA2
Images
HA721565_1.jpg Fig1: Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 16 kDa
Observed band size: 14 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721565_2.jpg Fig2: Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

Lane 1: Human liver tissue lysate (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (40 µg/Lane)
Lane 3: Mouse spleen tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 16 kDa
Observed band size: 14 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HA721565_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.