Phospho-Tau (T231) Recombinant Rabbit Monoclonal Antibody [PSH01-04]
cat.: HA721570
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: PSH01-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr231 of human Tau-F (P10636-8).
Positive control: Human brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue.
Subcellular location: Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue

1:1,000
1:1,000
1:200
Uniprot #: SwissProt: P10636-8 Human | P10637 Mouse | P19332 Rat
Alternative names: AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure, included
Images
HA721570_1.jpg Fig1: Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 50 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 35-100 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721570) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721570_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721570_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721570_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721570_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Phospho-Tau (T231) with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721570, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.