XLF Recombinant Rabbit Monoclonal Antibody [PSH01-07]
cat.: HA721572
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH01-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Recombinant protein within human XLF aa 1-250 / 299.
Positive control: HepG2 cell lysate, 293T cell lysate, NCCIT cell lysate, HeLa cell lysate, Jurkat cell lysate, human breast tissue, human colon tissue, human kidney tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:1,000
Uniprot #: SwissProt: Q9H9Q4 Human
Alternative names: Cernunnos FLJ12610 NHEJ 1 Nhej1 NHEJ1, S. cerevisiae, homolog of NHEJ1_HUMAN Non homologous end joining factor 1 Non-homologous end-joining factor 1 Nonhomologous end joining factor 1 OTTHUMP00000164168 OTTHUMP00000206275 OTTHUMP00000206279 Protein cernunnos XLF XRCC4 like factor XRCC4-like factor
Images
HA721572_1.jpg Fig1: Western blot analysis of XLF on different lysates with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: NCCIT cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (30 µg/Lane)
Lane 5: Jurkat cell lysate (30 µg/Lane)

Predicted band size: 33 kDa
Observed band size: 37 kDa

Exposure time: 2 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721572) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721572_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721572_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721572_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.