Fyn Recombinant Rabbit Monoclonal Antibody [JE42-22]
cat.: HA721577
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE42-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Fyn aa 25-74 / 537.
Positive control: Rat testis tissue lysate, Mouse testis tissue lysate, Human brain tissue lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T cell lysate, NIH/3T3 cell lysate.
Subcellular location: Cytoplasm, Nucleus, Cell membrane, Perikaryon.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P06241 Human | P39688 Mouse | Q62844 Rat
Alternative names: C syn protooncogene Fyn FYN oncogene related to SRC FGR YES FYN_HUMAN OKT3 induced calcium influx regulator P59 FYN p59-Fyn Protein tyrosine kinase fyn Proto oncogene tyrosine protein kinase fyn Proto-oncogene c-Fyn Proto-oncogene Syn Protooncogene Syn SLK Src like kinase Src yes related novel gene Src-like kinase Src/yes related novel SYN Tyrosine kinase p59fyn T Tyrosine kinase p59fyn(T) Tyrosine-protein kinase Fyn
Images
HA721577_1.jpg Fig1: Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

Lane 1: Rat testis tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Human brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 61 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721577_2.jpg Fig2: Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 61 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721577_3.jpg Fig3: Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 61 kDa
Observed band size: 59 kDa

Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.