Fyn Recombinant Rabbit Monoclonal Antibody [JE42-22]
cat.: HA721577
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE42-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Fyn aa 25-74 / 537. |
| Positive control: | Rat testis tissue lysate, Mouse testis tissue lysate, Human brain tissue lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Perikaryon. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P06241 Human | P39688 Mouse | Q62844 Rat |
| Alternative names: | C syn protooncogene Fyn FYN oncogene related to SRC FGR YES FYN_HUMAN OKT3 induced calcium influx regulator P59 FYN p59-Fyn Protein tyrosine kinase fyn Proto oncogene tyrosine protein kinase fyn Proto-oncogene c-Fyn Proto-oncogene Syn Protooncogene Syn SLK Src like kinase Src yes related novel gene Src-like kinase Src/yes related novel SYN Tyrosine kinase p59fyn T Tyrosine kinase p59fyn(T) Tyrosine-protein kinase Fyn |
Images
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Fig1:
Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Human brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Fyn KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 180 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.