FGFR4 Recombinant Rabbit Monoclonal Antibody [JE59-96]
cat.: HA721578
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE59-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FGFR4 aa 753-802 / 802. |
| Positive control: | HepG2 cell lysate, Huh7 cell lysate, SW480 cell lysate, Raji cell lysate, PANC-1 cell lysate, MCF7 cell lysate, Caco-2. |
| Subcellular location: | Cell membrane, Endosome, Endoplasmic reticulum; Secreted. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P22455 Human |
| Alternative names: | CD 334 CD334 CD334 antigen fc13h 10 fc13h10 Fgfr 4 FGFR-4 Fgfr4 FGFR4_HUMAN Fibroblast growth factor receptor 4 Hydroxyaryl protein kinase JTK 2 JTK2 MGC20292 Protein tyrosine kinase TKF Tyrosine kinase related to fibroblast growth factor receptor Tyrosylprotein kinase |
Images
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Fig1:
Western blot analysis of FGFR4 on different lysates with Rabbit anti-FGFR4 antibody (HA721578) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: SW480 cell lysate Lane 4: Raji cell lysate Lane 5: PANC-1 cell lysate Lane 6: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88/110 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721578) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of Caco-2 cells labeling FGFR4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721578, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.