CD30 Recombinant Rabbit Monoclonal Antibody [JE57-71]
cat.: HA721579
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE57-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD30 aa 546-595 / 595. |
| Positive control: | HDLM-2 cell lysate, human hodgkin lymphoma tissue. |
| Subcellular location: | Cell membrane; Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P28908 Human |
| Alternative names: | CD 30 CD30 CD30 antigen CD30L receptor Cytokine receptor CD30 D1S166E KI 1 KI 1 antigen Ki-1 antigen KI1 Lymphocyte activation antigen CD30 TNFRSF 8 Tnfrsf8 TNR8_HUMAN Tumor necrosis factor receptor superfamily member 8 |
Images
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Fig1:
Western blot analysis of CD30 on different lysates with Rabbit anti-CD30 antibody (HA721579) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: Raji cell lysate (negative) Lane 3: Daudi cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 120 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721579) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CD30 antibody (HA721579) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721579) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.