Phospho-Beta Catenin (S33 + S37) Recombinant Rabbit Monoclonal Antibody [JE59-59]
cat.: HA721580
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE59-59
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser33 and Ser37 of human β-catenin.
Positive control: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate.
Subcellular location: Cytoplasm
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat
Alternative names: b-catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289
Images
HA721580_1.jpg Fig1: Western blot analysis of Phospho-Beta Catenin (S33 + S37) on different lysates with Rabbit anti-Phospho-Beta Catenin (S33 + S37) antibody (HA721580) at 1/1,000 dilution.

Lane 1: 293T whole cell lysate
Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate
Lane 3: C6 whole cell lysate
Lane 4: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 85 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.