GluA4 Recombinant Rabbit Monoclonal Antibody [JE36-81]
cat.: HA721582
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE36-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 101 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GluA4 aa 853-902 / 902. |
| Positive control: | Mouse cerebellum tissue lysate, rat cerebellum tissue lysate, SH-SY5Y. |
| Subcellular location: | Cell membrane. Cell junction > synapse > postsynaptic cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P48058 Human | Q9Z2W8 Mouse | P19493 Rat |
| Alternative names: | AMPA 4 AMPA selective glutamate receptor 4 AMPA-selective glutamate receptor 4 AMPA4 GluA 4 GluA4 GluR 4 GluR D GluR-4 GluR-D GluR4 GLUR4C GLURD Glutamate receptor 4 Glutamate receptor ionotrophic AMPA 4 Glutamate receptor ionotropic GRIA 4 Gria4 GRIA4_HUMAN Ionotropic Glutamate receptor 4 |
Images
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Fig1:
Western blot analysis of GluA4 on different lysates with Rabbit anti-GluA4 antibody (HA721582) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 µg/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721582) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of SH-SY5Y cells labeling GluA4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721582, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.