iNOS Recombinant Rabbit Monoclonal Antibody [JE59-61]
cat.: HA721583
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE59-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human iNOS aa 1-300 / 1,153. |
| Positive control: | RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P35228 Human | P29477 Mouse |
| Alternative names: | HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2_HUMAN NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2 |
Images
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Fig1:
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (HA721583) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721583) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.