iNOS Recombinant Rabbit Monoclonal Antibody [JE59-61]
cat.: HA721583
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE59-61 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human iNOS aa 1-300 / 1,153. |
| Positive control: | RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate, RAW264.7 cells treated with 1μg/mL LPS for 6 hours. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: P35228 Human | P29477 Mouse |
| Alternative names: | HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2_HUMAN NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2 |
Images
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Fig1:
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (HA721583) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (HA721583) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (HA721583) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.