PF4 Recombinant Rabbit Monoclonal Antibody [JE31-48]
cat.: HA721584
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PF4 aa 52-101 / 101. |
| Positive control: | Human plasma lysates, Mouse spleen tissue lysate, Mouse platelets lysate, Rat spleen tissue lysate, human liver tissue, human bone marrow tissue, rat bone marrow tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P02776 Human | Q9Z126 Mouse | P06765 Rat |
| Alternative names: | C-X-C motif chemokine 4 Chemokine (C X C motif) ligand 4 Chemokine (CXC motif) ligand 4 chemokine ligand 4 CXCL 4 CXCL4 Iroplact MGC138298 Oncostatin A Oncostatin-A OncostatinA PF 4 PF-4 PF4 Pf4a Platelet factor 4 PLF4_HUMAN SCYB 4 SCYB4 short form Small inducible cytokine subfamily B Small inducible cytokine subfamily member 4 |
Images
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Fig1:
Western blot analysis of PF4 on human plasma lysates with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 11 kDa Observed band size: 14 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PF4 on different lysates with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate (30 µg/Lane) Lane 2: Mouse platelets lysate (6 µg/Lane) Lane 3: Rat spleen tissue lysate (30 µg/Lane) Predicted band size: 11 kDa Observed band size: 14 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PF4 antibody (HA721584) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-PF4 antibody (HA721584) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.