Cripto1 Recombinant Rabbit Monoclonal Antibody [JE48-67]
cat.: HA721586
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE48-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cripto1 aa 31-169. |
| Positive control: | NCCIT cell lysates. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P13385 Human |
| Alternative names: | CR CRGF Cripto 1 Cripto 1 growth factor cripto Cripto-1 growth factor Cripto1 growth factor Epidermal growth factor like cripto protein CR1 Epidermal growth factor-like cripto protein CR1 TDGF 1 TDGF1 TDGF1_HUMAN Teratocarcinoma derived growth factor 1 Teratocarcinoma-derived growth factor 1 |
Images
|
Fig1:
Western blot analysis of Cripto1 on NCCIT cell lysates with Rabbit anti-Cripto1 antibody (HA721586) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721586) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.