IFT88 Recombinant Rabbit Monoclonal Antibody [JE64-77]
cat.: HA721590
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IFT88 aa 1-50 / 824. |
| Positive control: | HEK-293 cell lysate, 293T cell lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13099 Human | Q61371 Mouse Entrez Gene: 305918 Rat |
| Alternative names: | D13S1056E DAF19 hTg737 Ift88 IFT88_HUMAN Intraflagellar transport 88 homolog Intraflagellar transport protein 88 homolog MGC26259 Polaris homolog Probe hTg737 (polycystic kidney disease, autosomal recessive) Recessive polycystic kidney disease protein Tg737 homolog RP11-172H24.2 Tetratricopeptide repeat domain 10 Tetratricopeptide repeat protein 10 TG737 TPR repeat protein 10 TTC10 |
Images
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Fig1:
Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA721590) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (40 µg/Lane) Lane 4: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721590) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.