KIF4A Recombinant Rabbit Monoclonal Antibody [JE38-91]
cat.: HA721591
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE38-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 140 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KIF4A aa 31-80 / 1,232. |
| Positive control: | HeLa cell lysate, U-87 MG cell lysate, 293T cell lysate, SW480 cell lysate, mouse thymus tissue lysate, NIH/3T3 cell lysate, human lung carcinoma tissue. |
| Subcellular location: | Nucleus matrix, Cytoplasm, cytoskeleton, spindle, Midbody, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: O95239 Human | P33174 Mouse |
| Alternative names: | Chromokinesin Chromokinesin-A Chromosome associated kinesin KIF4A Chromosome-associated kinesin KIF4A FLJ12530 FLJ12655 FLJ14204 FLJ20631 HSA271784 KIF4 KIF4 G1 KIF4A KIF4A_HUMAN KIF4G1 Kinesin family member 4A |
Images
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Fig1:
Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (HA721591) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: SW480 cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (HA721591) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-KIF4A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 60 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-KIF4A antibody (HA721591) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721591) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.