PSMA Recombinant Rabbit Monoclonal Antibody [JE42-45]
cat.: HA721593
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE42-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMA aa 1-150 / 750. |
| Positive control: | LNCaP cell lysate, 22RV1 cell lysate, human prostate carcinoma tissue. |
| Subcellular location: | Cell membrane; Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q04609 Human |
| Alternative names: | Cell growth inhibiting protein 27 Cell growth-inhibiting gene 27 protein FGCP Folate hydrolase (prostate-specific membrane antigen) 1 Folate hydrolase 1 Folate hydrolase Folate hydrolase prostate specific membrane antigen 1 FOLH 1 FOLH Folh1 FOLH1_HUMAN Folylpoly gamma glutamate carboxypeptidase Folylpoly-gamma-glutamate carboxypeptidase GCP 2 GCP II GCP2 GCPII GIG27 Glutamate carboxylase II Glutamate carboxypeptidase 2 Glutamate carboxypeptidase II Membrane glutamate carboxypeptidase mGCP N acetylated alpha linked acidic dipeptidase 1 N-acetylated-alpha-linked acidic dipeptidase I NAALAD 1 NAALAD1 NAALAdase NAALADase I Prostate specific membrane antigen Prostate specific membrane antigen variant F Prostate-specific membrane antigen PSM PSMA Pteroylpoly gamma glutamate carboxypeptidase Pteroylpoly-gamma-glutamate carboxypeptidase |
Images
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Fig1:
Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.