MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]
cat.: HA721595
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MT-ND1 aa 23-67 / 318. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse smooth muscle tissue lysate. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P03886 Human | P03888 Mouse | P03889 Rat |
| Alternative names: | Complex I, subunit ND1 Mitochondrially encoded NADH dehydrogenase 1 MT-ND1 MTND1 NAD1 NADH dehydrogenase subunit 1 (complex I) NADH dehydrogenase subunit 1 NADH-ubiquinone oxidoreductase chain 1 NADH-ubiquinone oxidoreductase, subunit ND1 NADH1 ND1 NU1M_HUMAN |
Images
|
Fig1:
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 36 kDa Observed band size: 37 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lane 3: Mouse smooth muscle tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 37 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.