POLD1 Recombinant Rabbit Monoclonal Antibody [JE35-73]
cat.: HA721597
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human POLD1 aa 1,001-1,050 / 1,107. |
| Positive control: | HeLa cell lysate, A549 cell lysate, 293T cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-3M cell lysate, mouse testis tissue lysate, rat testis tissue lysate, SW480 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P28340 Human | P52431 Mouse | O54747 Rat |
| Alternative names: | Polymerase (DNA directed) delta 1 catalytic subunit CDC2 CDC2 homolog CRCS10 DNA directed DNA polymerase delta 1 DNA directed polymerase delta 1 DNA pol delta 1 DNA polymerase delta catalytic subunit DNA polymerase subunit delta p125 DPOD1_HUMAN MDPL POLD POLD 1 POLD1 Polymerase (DNA directed) delta 1 catalytic subunit 125kDa Polymerase (DNA) delta 1 catalytic subunit Polymerase DNA directed delta 1 catalytic subunit 125kD polymerase, DNA, delta |
Images
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Fig1:
Western blot analysis of POLD1 on different lysates with Rabbit anti-POLD1 antibody (HA721597) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-3M cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Lane 9: SW480 cell lysate (20 µg/Lane) Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721597) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-POLD1 antibody (HA721597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.