Biotin Conjugated Mouse IL-9 Recombinant Rabbit Monoclonal Antibody [PS01-82]
cat.: HA721605
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PS01-82 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-9 aa 19-144 (HA210631). |
| Positive control: | Recombinant Mouse IL-9 protein (HA210631). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-81] to Mouse IL-9 antibody (Capture) (HA721603) and Recombinant Mouse IL-9 protein (HA210631) as the standard. The reference range value is 7.81-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P15247 Mouse Entrez Gene: 16198 Mouse Unigene: 3006 Mouse |
| Alternative names: | Cytokine P40 Homolog of mouse T cell and mast cell growth factor 40 HP40 IL 9 IL-9 Il9 IL9_HUMAN Interleukin 9 Interleukin-9 Mast cell growth factor MCGF Megakaryoblast growth factor P40 p40 cytokine p40 T cell and mast cell growth factor T cell growth factor 3 T cell growth factor p40 T-cell growth factor P40 TCGF 3 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-9 matched pair antibodies Capture: HA721603, Mouse IL-9 Rabbit mAb [PS01-81] Detector: HA721604, Mouse IL-9 Rabbit mAb [PS01-82] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721603) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-9 protein (HA210631) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721604, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.