Biotin Conjugated Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-92]
cat.: HA721608
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PS01-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL17A 26-158. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q62386 Mouse Entrez Gene: 16171 Mouse |
| Alternative names: | CTLA 8 CTLA-8 CTLA8 cytotoxic T lymphocyte associated antigen 8 Cytotoxic T lymphocyte associated protein 8 Cytotoxic T lymphocyte associated serine esterase 8 Cytotoxic T-lymphocyte-associated antigen 8 IL 17 IL 17A IL-17 IL-17A IL17 IL17_HUMAN Il17a Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) interleukin 17A Interleukin-17A interleukin17 Interleukin17A OTTHUMP00000016597 OTTMUSP00000046003 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721608, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.