Biotin Conjugated Rat IL-2 Recombinant Rabbit Monoclonal Antibody [PS01-89]
cat.: HA721614
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PS01-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat IL-2 aa 21-155 (HA210683). |
| Positive control: | Recombinant Rat IL-2 protein (HA210683). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-88] to Rat IL-2 (Capture) (HA721612) and Recombinant Rat IL-2 protein (HA210683) as the standard. The reference range value is 109.38-14,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P17108 Rat Entrez Gene: 116562 Rat Unigene: 9871 Rat |
| Alternative names: | Aldesleukin IL 2 IL-2 IL2 IL2_HUMAN Interleukin 2 Interleukin-2 interleukin2 Involved in regulation of T cell clonal expansion Lymphokine OTTHUMP00000164090 POIL2 T Cell Growth Factor T-cell growth factor TCGF |
Images
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Fig1:
Sandwich ELISA analysis of Rat IL-2 protein matched pair antibodies Capture: HA721612, Rat IL-2 Rabbit mAb [PS01-88] Detector: HA721613, Rat IL-2 Rabbit mAb [PS01-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721612) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IL-2 protein (HA210683) starting from 14,000 pg/mL to 0 pg/mL and detect antibody (HA721613, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.