Rat IFN gamma Recombinant Rabbit Monoclonal Antibody [PS01-96]
cat.: HA721618
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PS01-96 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat IFN gamma aa 23-156 (HA210577). |
| Positive control: | Recombinant Rat IFN gamma protein (HA210577). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-97] to Rat IFN gamma (Detecor) (HA721619) and Recombinant Rat IFN gamma protein (HA210577) as the standard. The reference range value is 156.25-10,000 pg/mL. |
| Uniprot #: | SwissProt: P01581 Rat |
| Alternative names: | IF 1 IFG IFI ifn g IFN gamma IFN immune IFN, immune IFN-gamma IFNG IFNG_HUMAN Immune interferon Interferon gamma Type II Interferon |
Images
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Fig1:
Sandwich ELISA analysis of Rat IFN gamma protein matched pair antibodies Capture: HA721618, Rat IFN gamma Rabbit mAb [PS01-96] Detector: HA721619, Rat IFN gamma Rabbit mAb [PS01-97] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721618) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IFN gamma protein (HA210577) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (HA721619, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.