FADD Recombinant Rabbit Monoclonal Antibody [PSH01-13]
cat.: HA721625
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FADD aa 1-208 / 208. |
| Positive control: | HeLa cell lysate, A431 cell lysate, MCF7 cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, SK-Br-3 cell lysate, A431. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q13158 Human |
| Alternative names: | FADD FADD protein FADD_HUMAN Fas (TNFRSF6) associated via death domain Fas associated via death domain Fas associating death domain containing protein Fas associating protein Fas associating protein with death domain Fas TNFRSF6 associated via death domain FAS-associated death domain protein FAS-associating death domain-containing protein GIG 3 GIG3 Growth inhibiting gene 3 protein Growth-inhibiting gene 3 protein H sapiens mRNA for mediator of receptor induced toxicity Mediator of receptor induced toxicity MGC8528 MORT 1 MORT1 Protein FADD |
Images
|
Fig1:
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA721625) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: HepG2 cell lysate Lane 6: Jurkat cell lysate Lane 7: SK-Br-3 cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 23 kDa Observed band size: 28 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721625) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of A431 cells labeling FADD with Rabbit anti-FADD antibody (HA721625) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (HA721625) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of A431 cells labeling FADD. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721625, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.