TopBP1 Recombinant Rabbit Monoclonal Antibody [PSH01-14]
cat.: HA721626
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 171 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TopBP1 aa 1,201-1,522 / 1,522. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, Jurkat cell lysate, K-562 cell lysate, HUVEC cell lysate, human testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle pole, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:200 |
| Uniprot #: | SwissProt: Q92547 Human |
| Alternative names: | DNA topoisomerase 2 binding protein 1 DNA topoisomerase 2-binding protein 1 DNA topoisomerase II binding protein 1 DNA topoisomerase II binding protein DNA topoisomerase II-beta-binding protein 1 DNA topoisomerase II-binding protein 1 DNA topoisomerase IIbeta binding protein 1 Hypothetical protein KIAA0259 [Fragment] KIAA0259 TOP2BP1 TOPB1_HUMAN TOPBP 1 TopBP1 Topoisomerase (DNA) II binding protein 1 Topoisomerase II binding protein 1 |
Images
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Fig1:
Western blot analysis of TopBP1 on different lysates with Rabbit anti-TopBP1 antibody (HA721626) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HCT 116 cell lysate Lane 4: Jurkat cell lysate Lane 5: K-562 cell lysate Lane 6: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721626) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TopBP1 on different lysates with Rabbit anti-TopBP1 antibody (HA721626) at 1/2,000 dilution. Lane 1: HEK-293-si NT cell lysate Lane 2: HEK-293-si TopBP1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721626) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TopBP1 antibody (HA721626) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721626) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.