SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15]
cat.: HA721627
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 181 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SMARCA2 aa 231-280 / 1,590. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, MCF7 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human prostate carcinoma tissue, MCF7. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P51531 Human |
| Alternative names: | ATP dependent helicase SMARCA2 ATP-dependent helicase SMARCA2 BAF190 BAF190B BRG1-associated factor 190B BRM FLJ36757 Global transcription activator homologous sequence hBRM hSNF2a MGC74511 Possible global transcription activator SNF2L2 Probable global transcription activator SNF2L2 Protein brahma homolog SMARCA2 SMCA2_HUMAN SNF2 alpha SNF2 like 2 SNF2-alpha SNF2/SWI2 like protein 2 SNF2L2 SNF2LA Sth1p Sucrose nonfermenting 2 like protein 2 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 |
Images
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Fig1:
Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (HA721627) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: MCF7 cell lysate Lane 5: COS-1 cell lysate Lane 6: NCCIT cell lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 181 kDa Observed band size: 200 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721627) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA721627) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721627) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA721627) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721627) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of MCF7 cells labeling SMARCA2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721627, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.